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Ultrapole, ultra-structural bio-imaging
Responsable : Jacomina Krijnse Locker • Responsable Tech. : Jacomina Krijnse Locker
Adresse : 28, rue du Dr. Roux
75015 Paris

complements

Certifications


Plate-forme certifiée ISO 9001

Site web

https://research.pasteur.fr/en/team/ultr...

Presentation de la plate-forme

We are a state-of-the-art electron microscopy platform for life sciences, our potential in terms of equipment and expert personnel is paralleled by only a few laboratories in Europe. We offer scientific and technical support in electron microscopy to study host-pathogen interactions as well as the pathogen itself at the ultra-structural level. In addition, we offer our expertise to external users that come with a wide variety of biological questions.
We offer pipelines from sample preparation, imaging to data analysis.
Sample preparation:
1. conventional room temperature embedding and room temperature sectioning
2. preservation by rapid freezing using plunge freezing or high-pressure freezing. Sectioning after freeze-substitution or by cryo-EM of vitreous sections
3. EM immuno-labeling according to Tokuyasu
4. Critical point drying and sputtering
Imaging:
1. Imaging of thin sections by transmission EM.
2. Automated 3D imaging by electron tomography or focused ion beam SEM at room temperature
3. Batch and array tomography
4. cryo-EM of single particles and cryo–electron tomography
5. correlative light- and electron microscopy/electron tomography
Data analysis:
Reconstruction of 3D data sets, manual and automated segmentation.

Moyens et equipements

Our equipment consists of modern sample preparation, imaging and data analyses machines.
Sample preparation:
Three microtomes for room temperature and two for cryo-sectioning one equipped for cryo-EM of vitreous sections.
One high-pressure freezer
Two freeze-substitution machines
One automated critical point dryer
Imaging:
Three transmission EMs that enable for automated imaging, one for high-resolution cryo-EM equipped with a Falcon II direct detector. All three are equipped for electron tomography acquisitions
One Jeol scanning EM equipped for cryo-SEM
One focused ion beam SEM for 3D data acquisition of large volumes
A cryo-light microscope (cryo-CLEM) for automated correlative light- and electron tomography.
Data analyses:
Three working stations for reconstructions of 3D data-sets (electron tomography tilt series and FIB-SEM data sets) and for manual and automated segmentation.

Galerie

Aucun visuels disponibles.