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Proteomics @ PSL Research University
Responsable : Co-directors: J. Vinh and D. Loew • Responsable Tech. : ESPCI VINH, 50% Institut Curie LOEW, 80%
Adresse : PSL Research University Proteomics Facility
(anciennement Protéomique Montagne Sainte Geneviève)
SMBP CNRS USR3149
ESPCI ParisTech
10 rue Vauquelin
75231 Paris cedex 05

complements

Certifications

Pas de certification ISO

Site web

https://www.smbp.espci.fr/&8208;Mass&820...

Presentation de la plate-forme

Facility
- protein digestion by trypsin, endo Lys-C, endo AspN, endo Glu-C (in-solution or in-gel) and glycosydases
- MALDI MS (fingerprinting) and MS/MS (sequencing)
- ESI and nanoESI FT MS and MS/MS
- nano LC MS/MS runs of proteolytic or endogenous peptides complex mixtures
- database searches (Mascot / Sequest / ProteinPilot / X!Tandem / Peaks / MaxQuant andromeda / Byonic)
- scientific and technical support for the CNRS national network TGE FTICR for high resolution mass spectrometry (FR3624, https://fticr.polytechnique.fr/)
- open access after training to MALDI instrument
- on-line access to the results through our home-made bioinformatics solution MyProMS (upon demand)

Collaborations
- protein modifications (phosphoproteome, ubiquitome, acetylome, redoxome, glycosylations, and other genetically encoded or chemical and metabolic modifications, etc)
- peptidomics
- large scale protein identification, proteome analysis, quantification (label-free, SILAC, TMT and iTraq, etc.)
- middle down and middle up, top down on FT MS
- glycomics

Moyens et equipements

In total on both sites: 3 nanoLC U3000 instruments, 5 nanoLC RSLC U3000 instruments are available.

ESPCI site:
- MALDI-TOF/TOF 5800 Applera, 2012 (ESPCI)
nanoESI Q Exactive HF, ThermoFisher, 2016 (CR Ile de France)
- nanoESI Q Exactive, ThermoFisher, 2011 (Paris, ESPCI, CR Ile de France)
- nanoESI LTQ Orbitrap, ThermoFisher, 2008 (Industrial partnership)
- nanoESI LTQ FT ThermoFisher ECD/IRMPD, 2003 (CPER FRT)

Institut Curie site
- MALDI-TOF/TOF 4800 plus AB SCIEX, 2008 (PROCAN)
- NanoESI Orbitrap tribrid Fusion ThermoFisher, 2014 (FRM, Cancéropôle Ile-de-France)
- NanoESI TripleTOF 6600 AB SCIEX, 2014 (ITMO, Cancéropôle Ile-de-France)

Galerie