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FBI-PICSL
Responsable : Pierre-François Lenne • Responsable Tech. : Cédric Matthews - IBDM -100% Sébastien Mailfert - CIML - 100% Artemis Kosta - IMM - 100%
Adresse : IBDM
Campus de Luminy
13288 Marseille Cedex 09
13009 Marseille

complements

Certifications


Plate-forme certifiée ISO 9001

Site web

http://www.picsl.univ-mrs.fr/

Presentation de la plate-forme

PICSL is a multi-site platform with three facilities, the CIML and IBDM on the Luminy campus and the IMM on the Joseph Aiguier Campus (at 15 min from the Luminy campus). Altogether, PICSL covers a large field of imaging technics, from electron microscopy to multiphoton microscopy. However, given the number of users (currently more than 800 users), some of the equipment (e.g. wide field and confocal microscopy) are present on the different sites. Moreover, given the nature of the scientific themes developed in the institutes, which host the facility, each site has its own specificity with equipment specifically designed for a set of applications directly linked to experimental constraints.
Note that, once having received and validated a specific training, users of the three facilities can book the microscopes online.
CIML site – The site has a strong experience with R&D activities (1) in Fluorescence Correlation Spectroscopy (FCS)-based methodology combining STED, confocal and lifetime imaging for a large variety of modalities of applications, and (2) in single molecule imaging for dynamic and static observations based on a homemade PALM/dSTORM set-up. The facility has also an expertise in imaging technics and analytical methods to explore the immune systems in whole organisms (C. elegans, Mus musculus). This includes imaging of immune organs by multispectral analyses, mouse embryos by light sheet microscopy, or dynamic molecular imaging in live cells by higher resolution (AirScan) microscopy.
IBDM site – The site has developed expertise in light sheet microscopy for fast and long-term imaging of whole organisms (C. elegans, Drosophila) and super-resolution optical microscopy. The latter is implemented with a commercial STED microscopy and a PALM/STORM set-up developed on-site for imaging of whole embryos. The EM facility hosted by the IBDM is currently has an expertise in advanced imaging modes such as electron tomography (ET), Scanning Transmission Electron Microscopy (STEM), Scanning Electron Microscopy in « Serial Block-Face » mode, Correlative Light and Electron Microscopy (CLEM).
IMM site – The site masters a number of imaging techniques to explore a large variety of fundamental microbiology aspects with the constrain specific to the size of their models. The facility has a specific expertise mainly in cryo-electron microscopy (cryo-EM) but also in light microscopy with a structurated illumination microscope.

1) prestations

a) Microscopy methods utilizing contrast: DIC, phase contrast, interference contrast
microscopy by reflection, second harmonic generation
b) Fluorescence microscopy techniques: wide field, structured illumination microscope, confocal,
multi-photon, spinning disk, Macro-confocal
c) Live cell time lapse microscopy (environmentally controlled) visible and fluorescence
d) Nano-ablation
e) High resolution microscopy with STED and PALM/dSTORM or with airy scan
f) Light sheet microscopy for in toto acquisition and lattice light sheet
g) F-techniques : FRAP, ratiometric-FRET, FCS, FCCS
h) polarisation detection with spinning disk
i) Deconvolution with and 4 D reconstruction with Huygens algorithmes
j) Specialized image analyses with Image J, Metamorph, ICY, matlab and Imaris softwares
k) homemade software for fast single particle tracking and single molecule localization
J) OMERO Database for images

1.2) electron microscopy

-Sample Preparation for EM
=> Chemical fixation
=> High pressure freezing
=> Tokuyasu
=> Plunge freezing
=> Cryosubstitution

- Morphological studies in EM
=> Transmission
=> Scanning electron microscopy

- Immunocytochesmitry for TEM
=> Preemmedding
=> Postemmebedding
=> Tokuyasu technics

- Tridimensional EM technics
=> Electron tomography

- Combine optic and electron microscopy
=> CLEM (Correlative Light Electron Microscopy)

- Serial Block Face Scanning Electron Microscopy

Moyens et equipements

A summary of the resources/services offered by the PICL infrastructure is provided below:
- Light and fluorescence microscopy:
Wide field microscopes (3 at CIML; 3 at IBDM)
Confocal, SIM and spinning disk microscopes (4 at CIML; 9 at IBDM, 2 at IMM)
Multiphoton microscopes (1 at CIML; 2 at IBDM)
Light sheet microscopes (1 at CIML; 1 at IBDM)
High resolution microscopes - STED, PALM/dSTORM - (2 at CIML; 2 at IBDM)
Specialized techniques - FCS, Polarization-resolved imaging, optical tweezers, SHG imaging - (3 at CIML; 1 at IBDM, 1 at IMM)
- Electron microscopy:
TEM FEI Tecnai G2 (200KV) (IBDM)
TEM FEI MORGAGNI (120KV) (IBDM)
SEM FEI TENEO VS (IBDM)
TEM FEI Tecnai G2 20 TWIN (200KV), Gatan Oneview camera (4Kx4K) (IMM)
SEM Tescan VEGA3 SBU, Low-Vacuum (IMM)

- Samples preparation devices – The IBDM and IMM facilities have the equipment for the preparation of samples by conventional techniques and by cryo-techniques.

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