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Pasteur - Cytometry and Biomarkes UTechS
Responsable : Milena Hasan • Responsable Tech. : Milena Hasan, Sophie Novault, Valentina Libri
Adresse : 25-28 rue du Dr Roux
75015 Paris

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https://research.pasteur.fr/fr/team/tech...

Presentation de la plate-forme

Cytometry and Biomarkers Unit of Technology and Service (CB UTechS) – Mission and Vision:
The CB UTechS is a well-established open-access platform that provides integrate support to translational, clinical and fundamental research projects through 28 state-of-the-art technologies. The core vision of the CB UTechS is to contribute to improving human health through a better understanding of human physiology and disease pathogenesis. The current research projects supported by the facility focus on infectious disease, cancer, allergy, autoimmunity, transplantation, and include a substantial number of projects with fundamental topics. The large portfolio of mutually complementary equipment within the CB UTechS ensures all technological solutions necessary for responding to a scientific question within a single facility. Our technologies are selected to permit cell phenotyping and sorting, molecular profiling and experimentation at single cell level. This unique setup, together with the long-lasting expertise of the team, makes the CB UTechS an immunophenotyping platform of excellence, ideal for running biomedical research projects.

The CB UTechS – Team and Activities:
The CB UTechS is directed by Milena Hasan, PhD, and has two strong teams of senior staff members with expertise in multiplex technical areas. The support to the user community is organised through two activities: (I) The Cytometry platform focuses on cellular immunophenotyping and single cell separation, by using multiple state-of-the-art technologies. The team is constituted of three experienced research engineers and two technicians. (II) The Biomarkers activity is focused on the discovery of biomolecules involved in physiological and pathological processes through different molecular approaches, including proteomics and transcriptomics. This core includes highly standardized medium- and high-throughput instrumentation for protein and RNA analysis and has a strong interest and expertise in single-cell studies. The team is constituted of four experienced research engineers. Both teams of the CB UTechS work tightly together and join their expertise on specific technologies to support biomedical research, and to provide a collaborative research space for the user community.

The CB UTechS - Bioinformatics:
Both singe-cell/single-molecule technologies and high-dimensional cytometers lead to an increased production of data and a necessity to provide tools to analyze them. To this end we have obtained user-licenses for selected data analysis software, making them available to the community. Thanks to the presence of two bioinformaticians, a personalized support, with project-tailored analysis of complex data generated by our technologies is available.

The CB UTechS – Laboratories & Technologies:
(1) A Bsl2 laboratory equipped by technologies for high-dimensional cytometry;
(2) A Bsl2 laboratory dedicated for cell sorting
(3) A Bsl2 laboratory for sample preparation and ultrasensitive protein quantification;
(4) A molecular biology laboratory, with clear separation of areas for manipulation of RNA, DNA, and for work with samples upon PCR amplification. It is equipped with all relevant instrumentation and related accessories (Bioanalyzer, Qubit, E-gel, PCR machine, centrifuges, refrigerators, sets of pipets, etc.);
(5) A Bsl2+ level laboratory for protein profiling by multiplex immunoassays
(6) A Bsl2+ level laboratory containing fully equipped cell culture facility (hypoxia workstation, 4 biosafety cabinets, incubators, centrifuges, freezers and refrigerators, all accessories), major cell phenotyping technologies for analysis of infected and/or human samples and a panel of instruments for single cell functional studies and single cell RNA profiling.
The technologies are accessible to all researchers at Institut Pasteur and to the worldwide scientific community.

Communication and visibility:
Our website (https://research.pasteur.fr/fr/team/technical-core/) is used to share the news and the relevant events with the scientific community. In order to insure a regular feedback from the user community and to keep the users updated on all news at the CB UTechS, we organize a yearly meeting with our users.

International Networking. Our website (https://research.pasteur.fr/fr/team/technical-core/) is used to share the news and the relevant events with the scientific community. In order to insure a regular feedback from the users and to keep them updated on all news at the CB UTechS, we hold an annual meeting with our user community.
The CB UTechS is an active member of FOCIS (The Federation of Clinical Immunology Societies). As the leader of the Institut Pasteur FOCIS Center of Excellence (FCE), we regularly participate in the international FOCIS meetings. Through the FOCIS, we have become a partner of the COST initiative, the European network of 13 FOCIS Centers of Excellence, and regularly participate in activities of European FCEs. Being an active FCE gives us the possibility to send two fellows per year to the annual course “The Advanced Course in Basic and Clinical Immunology” in Scottsdale, Arizona.
We are an active member of the CIMT (Association for Cancer Immunotherapy), and participate in CIMT-organized proficiency panels. Through our membership at the ISAC (International Society for Advanced of Cytometry) and the AFC (Association Française de Cytométrie), the team exchanges with the national and international community of the cytometrists and has an insight in evolution of technologies and their applications. We are also a part of international core technologies’ networks Core4Life and CTLS (Core Technologies for Life Sciences). Earlier this year, in the context of Core4Life, we have started a « single cell » transversal initiative that gathers colleagues from five European Institutions.

Moyens et equipements

The CB UTechS provides access to 28 technologies and all accessories required for their use. The protfolio of the principal instruments includes:
LSR II, BD (2009)
LSR Fortessa, BD (2011)
FACSAria III, BD (2011)
Astrios, Beckman Coulter (2012)
FACSAria III, BD (2012)
FACSAria III, BD (2014)
Ion Proton+PGM,Thermo (2014)
Spectral Analyzer, Sony (2014)
LSR Symphony, BD (2015)
Astrios, Beckman Coulter (2015)
Nanostring PrepStation and counter, Nanostring (2015)
CytoFlex, Beckman Coulter (2016)
Polaris, Fluidigm (2016)
10xChromium, 10xGenomics (2017)
Simoa, Quanterix (2017)
FACSAria Fusion, BD (2018)
ImageStreamMARKII, Luminex (2018)
FACSAria Fusion, BD (2019)
Xvivo, Biospherix (2019)

Galerie