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Responsable : Juliette Godin, ICI/Philippe Rondé, PIQ • Responsable Tech. : ICI: Bertrand Vernay, 100% PIQ: Ludovic Richert, 50%
Adresse : QUEST
1 rue Laurent Fries
67404 Illkirch-Graffenstaden



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Presentation de la plate-forme

QuESt : Quantum Efficiency Strasbourg
QuESt is the association of the two imaging facilities from the IGBMC (ICI) and the Faculty of Pharmacy, UMR7021 (PIQ), see the website The two partner facilities are closely located on the bio-campus of Illkirch and are only separated by a short 10 minutes walk. Each site is described in more details in section I-b.
The governance of QuEst is provided by its Steering Committee, for more details see section I-b.

Access to QuESt
QuESt is an open access facility catering for the scientific community, both academia and industry, and running a pay as you use policy. Users can directly contact both facilities to gain access.

Multi scale imaging from the molecule to the whole animal
QuESt offers a multi-scale imaging approach from the molecule to the whole animal thanks to its wide range of advanced commercial and custom-built optical systems:
- laser scanning and spinning disk confocal for FRAP, FRET, FLIM, FCS, photo-manipulation and photo-ablation
- multiphoton for intravital and large samples imaging
- light-sheet for in vivo imaging
- PALM/STORM and Live-SR for super-resolution microscopy
- scanning and transmission electron microscopy.
- correlative light-electron microscopy (CLEM)
- technological development with custom-built optical systems

Dedicated users training and support with a team of experts
All users receive extensive training to autonomously operate the microscope(s). The team of experts is dedicated to provide a high level of support and advices from samples preparation protocols to the final image processing and analysis steps. The multidisciplinary background of the QuESt engineers in physics/optics, cell biology, chemistry, informatics and image processing facilitates the interactions with the researches of varied horizons and is an asset for undertaking new instrumental and methodological developments in interaction with both academic and industry R&D laboratories.

Training in quantitative bio-images processing and analysis
Image processing and analysis has become a cornerstone of quantitative biological imaging thanks to technological development and a rich ecosystem of software both commercial and open-source. QuESt is currently offering 2 theoretical & practical courses:
- Introduction to image processing and analysis with ImageJ/Fiji (2019-2020, 80 trainees)
- Scripting and automation with the ImageJ macro language (2019-2020, 24 trainees)

Teaching and education
QuESt is a partner in the teaching programme of the Faculty of Pharmacy, Ecole Supérieure de Biotechnologie de Strasbourg (Strasbourg University), Strasbourg University and Telecom Strasbourg in the following courses:
- UE Basics techniques in microscopy and UE Advanced microscopy techniques
- Engineering degree in biotechnology
- Master high throughput in life sciences, Master synthetic biology, Master Imaging, Robotics, Engineering for the Life Sciences and Master Cell Physics Strasbourg
- DU Quantitative Microscopy

Moyens et equipements

QuESt-ICI site:
List of instruments
i) imaging:
- 2 transmission electron microscopes (2005, 2008);
- 1 scanning electron microscope (1994);
- 2 widefield epifluorescent macroscopes;
- 1 confocal macroscope (developed in 2007);
- 6 widefield epifluorescent microscopes;
- 2 video-microscopes;
- 8 confocal microscopes (3 multiphoton, 2009-2015);
- 2 spinning-disk microscopes (2012, 2018);
- 1 light-sheet microscope (2015).

ii) sample preparation
- 1 laminar flow hood;
- 3 aspiration hoods;
- 5 cell culture incubators;
- 2 freeze-substitution devices;
- 1 knife-maker;
- 3 ultramicrotomes;
- 3 brightfield microscopes;
- 3 brightfield binoculars;
- 1 epifluorescent binocular.

iii) micromechanics (major equipment)
- Computerized numerical control (CNC) turning center (3 axes);
- CNC drill for plastic materials;
- Precision lathe with 3-jaw chuck and live centre;
- Grinder;
- 3D-CAD workstation;

iv) image processing:
- 4 workstations equipped with image processing software (e.g. Imaris, Metamorph)

QuESt-PIQ site:
- CW-STED microscope Olympus IX81 (2012)
- Spectral confocal microscope Leica TCS SPE II (2012)
- Single particle microscopy (SM, FRET sPT) (2010),with an adaptation of upconverting nanoparticles (UCNP) with a monophton exciation in the IR (2015)
- 3D-PALM microscope Nikon Ti-E (PALM, dSTORM, sPAINT) (2016) with the adaptive optics modulus, this instrument was a spin-off of SM microscope.
- Multimodal microscope iMIC Till Photonics (FRET / FRAP / TIRF / Structured Illumination / dSTORM) (2009)
- Atomic Force Microscopy NT-NDT Prima (2008)
- Videomicroscope Leica AS MDW for fast 4D recording (2008)
- Videomicroscope Leica DM IRBE2 coupled with a micro-injection system (Eppendorf) (2008)
- Quantitative imaging system 2 photons Olympus (FLIM / FCS) (2006). This set-up is continuously upgraded. In 2013, a broad-band Insight two photon laser was implemented
- Correlative AFM-fluorescence microscopy NT-MDT Aura (2011)
- A cell culture room exclusively dedicated to users of the platform
- 4 offline stations
- a data server in RAID 5