Cytometry and Biomarkes UTechS
Responsable : Milena Hasan • Responsable Tech. : Milena Hasan
Adresse : 25-28 rue du Dr Roux
Plate-forme certifiée ISO 9001
Presentation de la plate-forme
Cytometry and Biomarkers Unit of Technology and Service (CB UTechS) – Mission and Vision:
The CB UTechS is a state-of-the art technology platform conceived to support translational and fundamental research projects. The mission of the CB UTechS is to support the community of clinically-oriented scientists and research-oriented clinicians on the Institut Pasteur, in neighboring hospitals and collaborating scientific institutions by providing access, training, advice, service, support and collaborations on our technologies and related software, to develop new protocols and pipelines and to help with data analysis. The core vision of the CB UTechS is to improve human health through a better understanding of human physiology and disease pathogenesis. The current research projects supported by the Facility focus on infectious diseases, cancer, allergy, autoimmunity and transplantation, but include also a substantial number of projects with fundamental topics.
The CB UTechS broad spectrum of available technologies and technical expertise built around the equipment makes the CB UTechS the leading translational research technology platform in the region.
The CB UTechS – Team and Activities:
The CB UTechS has two strong teams of senior staff members with expertise in multiplex technical areas, who provide support to the user community through two activities: (I) The Cytometry activity has a focus on cellular immunophenotyping and single cell separation. This includes standard technologies and innovative instruments (e.g., imaging cytometer, spectral analyser, ViroFACS). The team is constituted of three experienced research engineers and two technician. (II) The Biomarker activity is focused on biomarker discovery, proteomics and transcriptomics. This core includes highly standardized medium- and high-throughput instrumentation for protein and RNA analysis and has a strong interest and expertise in single-cell studies. The team is constituted of three experienced research engineers and one bioinformatician.
Both teams of the CRT-TC work tightly together and join their expertise on specific technologies to support biomedical research on campus and in the community, and to provide a collaborative research space for the community of users.
The CRT Technology Core - Bioinformatics:
Both singe-cell/single-molecule technologies and high-dimensional cytometers lead to an increased production of data and a necessity to provide tools to analyze them. To this end we have obtained user-licenses for selected data analysis software, making them available to the community. In addition, we propose adapted bioinformatic support for the analysis of all data generated by our technologies.
CRT-Technology Core – Laboratories & Technologies:
In order to allow efficient support to biomedical and fundamental research projects, we have installed our technologies in six dedicated laboratories so that all requirements of a project can be met within the Facility:
(1+2) High-end cell phenotyping and sorting technologies are available in two fully-equipped laboratories of the P2 level;
(3) A laboratory equipped by a chemical hood is dedicated to RNA extraction;
(4) Molecular biology laboratory is designed to allow clear separation of areas for manipulation of RNA, of DNA and for work with samples upon PCR amplification. It is equipped by all relevant instrumentation and related accessories (centrifuges, refrigerators, sets of pipets, bioanalyzer, PCR machine, e-gel, Qubit);
(5+6) Two laboratories of Bsl2+ level host the protein analysis platform (Bioplex200 + DropArray), technologies for single cell functional studies and RNA/DNA preparation (C1, Polaris), a fully equipped cell culture facility (3 biosafety cabinets, incubators, centrifuges, all accessories) and a set of cell phenotyping technologies dedicated for analysis of infected and/or human samples (FACSAria, ViroFACS, LSR, Seahorse, Incucyte, imaging cytometer, Spectral analyzer);
The technologies are accessible to all researchers at Institut Pasteur and worldwide scientific community. An autonomous access after dedicated training by the staff of the CRT-TC is proposed for a major part of the technologies. The team proposes help with experimental design, modification of protocols and pipelines in sample preparation and in data analysis.
Communication and visibility:
Several actions have been engaged to increase the visibility of the CRT-TC resources and activities. Our website (https://research.pasteur.fr/fr/team/technical-core/) is used to share the news and the relevant events with the scientific community. In order to insure a regular feedback from the user community and to keep the users updated on all news at the CRT-TC, we organize a yearly meeting with our users. A general presentation of the CRT-TC activities is organized for each generation of candidates for Pasteur Paris University International Doctoral Program (PPU), for HOMIN EU International PhD Training Network and for the students enrolled in the Master 2 course Frontiers in Biological Psychiatry.
The CRT-TC is an active member of FOCIS (standing for The Federation of Clinical Immunology Societies, see www.focisnet.org). As a FOCIS Center of Excellence (FCE), we regularly participate to international meetings of FOCIS. Through the FOCIS, the CRT-TC has become a partner of COST initiative, the European network of 13 FOCIS Centers of Excellence and regularly participates to the activities of European FCEs. In October 2016, the CRT-TC has hosted the first European FOCIS Advanced Course in Basic and Clinical Immunology. Being an active FCE offers us the possibility of sending two fellows per year to the annual course, The Advanced Course in Basic and Clinical Immunology in Scottsdale, Arizona.
We are an active member of the CIMT (Association for Cancer Immunotherapy), and participate in CIMT-organized proficiency panels. Through a membership of the ISAC (International Society for Advanced of Cytometry) and AFC (Association Française de Cytométrie), the team exchanges with the national and international community of the cytometrists and has an insight in evolution of technologies and their applications.
Moyens et equipements
The Facility provides access to 30 technologies and all accessories required for their use. Among these technologies, the following have a value of >150K€:
FACSAria II, BD (2007)
FACSAria III, BD (2011)
FACSAria III, BD (2012)
FACSAria III, BD (2014)
LSR II, BD (2009)
LSR Fortessa, BD (2011)
LSR Symphony, BD (2015)
CytoFlex, Beckman Coulter (2016)
Spectral Analyzer, Sony (2014)
Astrios, Beckman Coulter (2012)
Astrios, Beckman Coulter (2015)
ViroFACS, BD (2017)
ImageStreamX, Thermo (2009)
Nanostring PrepStation and counter, Nanostring (2015)
Ion Proton+PGM,Thermo (2014)
iSCAN, Illumina (2013)
Polaris, Fluidigm (2016)
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